Kinetic studies of Bacillus polymyxa nitrogenase
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چکیده
منابع مشابه
Hydrogenase and nitrogenase in cell-free extracts of Bacillus polymyxa.
Grau, F. H. (University of Wisconsin, Madison), and P. W. Wilson. Hydrogenase and nitrogenase in cell-free extracts of Bacillus polymyxa. J. Bacteriol. 85:446-450. 1963.-Washed cells of Bacillus polymyxa strain Hino, treated with lysozyme, yield cell-free extracts that rapidly evolve hydrogen from reduced methyl viologen, formate, and pyruvate. Hydrogenase is particulate, 86% being sedimented a...
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The endospore of Bacillus polymyxa was originally described by Meyer (1897) in a study of a bacillus which he designated Astasia asterospora. Meyer's drawings of the spore, showing ridges and the star shaped aspect, were reproduced by Knaysi (1951) over the name Bacillus asterosporus. It has not been generally recognized that this name is synonymous with B. polymyxa (Smith, et al., 1952). So th...
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Pathways of ammonia assimilation into glutamic acid and alanine in Bacillus polymyxa were investigated by "N NMR spectroscopy in combination with measurements of the specific activities of glutamate dehydrogenase, glutamine synthetase, glutamate synthase, alanine dehydrogenase, and glutamic-alanine transaminase. Ammonia was found to be assimilated into glutamic acid predominantly by NADPH-depen...
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Four enzymes were separated from an extracellular preparation of Bacillus polymyxa by carboxymethylcellulose column chromatography. The pH optima were 8.3 to 8.5, 8.7 to 8.9, 9.2 to 9.4, and 9.5 to 9.6. All of the enzymes required calcium ion for maximum activity, whereas strontium ion was only partially effective in stimulating activity. Cobalt was the only other cation tested which was effect...
متن کاملTransformation of Bacillus polymyxa with plasmid DNA.
A plasmid transformation system was developed for Bacillus polymyxa ATCC 12321 and derivatives of this strain. The method utilizes a penicillin-treated-cell technique to facilitate uptake of the plasmid DNA. Low-frequency transformation (10(-6) per recipient cell) of plasmids pC194, pBD64, and pBC16 was accomplished with this method. Selection for the transformants was accomplished on both hype...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1976
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.126.2.743-750.1976